General Cell Based Assays
Revvity Health Sciences Inc AlphaLISA Human IL-8 High Performance Detection Kit, 500 Assay Points
AlphaLISA Human IL-8 High Performance Detection Kit, 500 Assay Points
Abcam Lipid Assay Kit (unsaturated fatty acids)
Lipid Assay Kit (unsaturated fatty acids)
The product is subject to the following: Abcam Restricted Use Statement
ETON BIOSCIENCE INC L-LACTATE ASSAY KIT 5ML
Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH. The Lactate Assay Kit is based on the reduction of the terazolium salt INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm.
Perkin Elmer US LLC UATR Sample Position Plate - 3 mm
The PerkinElmer Universal ATR (UATR) Accessory is ideal for the general IR analysis of solids and liquids. The UATR comes in a variety of crystal configurations as well as single/multiple reflections capabilities. It produces high quality spectra through the use of a pressure arm. The pressure arm force indicator ensures sample-to-sample and operator-to-operator reproducibility. The accessory is recognized when it is placed in the spectrometer's sample compartment, the optics are automatically optimized, and the system is ready to be used without additional alignment. This UATR is compatible with the Spectrum One/100/400/65 and Spectrum 3/Frontier spectrometers.
Cayman Chemical HDAC Fluorometric Activity As
Cayman’s HDAC Fluorometric Activity Assay Kit provides a fast, fluorometric method for measuring HDAC activity without the use of radioactivity, extraction, or chromatography. In the first step, an acetylated lysine substrate is incubated with samples. Deacetylation sensitizes the substrate such that treatment with the HDAC developer in the second step releases a fluorescent product. Trichostatin A is included as a control inhibitor. The fluorophore can be EAsily analyzed using a fluorescence plate reader with an excitation wavelength of 340-360 nm and an emission wavelength of 440-465 nm. The assay can be used for quantifying Class I and II HDAC activity from various sources.
Cayman Chemical LDL Uptake CellBased Assay Ki
LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific antibody and a Dylight™ 488-conjugated secondary antibody are included in the kit for identifying the distribution of LDL receptors. The kit provides a convenient tool for studying LDL uptake and its regulation at the cellular level.
Lonza Walkersville ToxiLight Non-Destructive Cytotoxicity BioAssay Kit with Plates, 500 Test
The ToxiLight BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. AK is a robust protein present in all eukaryotic cells, which is released into the culture medium when cells die. The enzyme actively phosphorylates ADP to form ATP and the resultant ATP is then measured using the bioluminescent firefly luciferase reaction. As the level of cytolysis increases, the amount of AK in the supernatant also increases, which results in emission of higher light intensity by the ToxiLight reagent. Because the ToxiLight BioAssay Kit exploits the fact that AK is released from cells when they die, there is no need for cell lysis (unlike many other cytotoxicity assays). Repeated samples of supernatant can therefore be taken over time without disrupting the cells themselves. This allows for kinetic analysis of cell death.
STEMCELL Technologies Busulfan, Size: 100 g
Inhibit proliferation of cancer cells by creating DNA breaks and DNA crosslinks with busulfan (Iwamoto et al.). Highly potent and cell-permeable busulfan forms interstrand crosslinks between the DNA bases guanine and adenine and between guanine and guanine (Ponti et al.). DNA breakage induced by busulfan-mediated DNA crosslinking induces senescence in cells (Chen et al.).
BioVendor, LLC QuickZyme Hydroxyproline Assay Kit 5 X 96-Assays (for collagen analysis)
QuickZyme Hydroxyproline Assay Kit, 5 x 96-Assays. Quantitative hydroxyproline measurement for collagen analysis. The ONLY hydroxyproline-based assay that does NOT require a drying step following acid hydrolysis of collagen, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Broad application: conditioned media, cells/cell extracts, tissues/tissue extracts. Species & collagen type independent. Dynamic range: 6-300 uM. Sensitivity: 2.4 uM. Sample vol. 5-35 ul. Measures total hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all types of collagen present without discriminating between the types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.
Abcam Lipase Assay Kit III (Fluorometric)
Lipase Assay Kit III (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam ATPase Assay Kit (Colorimetric)
ATPase Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Citrulline Assay Kit (Fluorometric)
Citrulline Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
